Expression and Subcellular Localization of Endocannabinoid System in Primary Interstitial Glandular Cells In Situ of Mice Ovary

Endocannabinoid synthesizing 2-AG and cannabinoid receptors have been reported in some organ of reproductive system oocyte ovary, but little is known about the expression subcellular localization CB1 DAGL-α interstitial gland tissue mouse ovary. The aim this study was to explain differential distribution endocannabinoid glandular cell DAGL-α-immunoreactivities were detected within cells, which are consisted smaller mitochondria with non-tubular cristae fewer lipid droplets glands. Their immunoreactivities localized association small vesicle clustering dispersed throughout cytoplasm. identity immunoreactive cells glands showed a possibility co-existence steroidogenic autocrine or paracrine exertion eCB signaling as well involvement regulation differentiation discussed.
 HIGHLIGHTS
 
 2-arachidonoyl glycerol (2-AG, endocannabinoid)-synthesizing enzyme (DAGL-α) its receptor localize primary mice
 first time for DAGL isozymes co-localize by Immuno-DAB-electron-microscopy. new knowledge represents locate intracellular vesicles. This suggests that had function through paracrine/autocrine released, already oocyte, neurons other cells
 tissues eCBs molecules may be plays unique functions steroidogenesis
 GRAPHICAL ABSTRACT